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Vacuum would only be present in the dipstick if the PCV/CCV system was working correctly. If a PCV port on the valve cover were disconnected (say on an engine with a traditional PCV setup) from the intake manifold and capped off then the engine would be creating positive pressure. The PCV relieves crankcase pressure with a controlled vacuum leak via the PCV system.

My old Volvo 240 kept blowing the dipstick out of the tube because the oil separator box was clogged and did not allow any pressure to be released into the intake. Volvo has their own version of a PCV setup as well but its the same principle.
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